FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2894246
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12750.044.6114278
CTGTCGC15600.035.999739
GTCTCGC14750.035.1533931
GTCTTGC27850.034.9088331
TCGCTCT15350.033.3026774
CGTTTCG1551.5477981E-632.5158849
CTCGCTC16350.032.1537673
TCTCGCT16550.031.3300632
CGCTCTG17750.027.1772585
GTGTTAG20200.025.6689381
GTATAAT8850.024.4120791
GTATTAG9400.023.7498361
TTGCTCT40900.023.5890644
TTATACT9900.023.2725524
CTTGCTC37900.023.1818123
TCTTGCT42700.022.5997522
GTACTAA7500.022.0847931
CTGTTGC31950.021.858999
GTATAGA9000.021.6046891
GTATAAG8700.021.5219121
Produced by FastQC (version 0.11.5)