FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2894246
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12500.039.743368
GTCTCGC13100.037.9650341
TCGCTCT15350.033.30244
CGCTCTG15550.031.948045
GTCTTGC29400.030.6456361
CTCGCTC16050.029.6073
CGTTTCG1255.369841E-428.7995369
GGTATAC6500.027.7225151
TCTCGCT17700.026.869772
GTATATA12400.026.7388111
CTGTCGC18800.026.0421339
GTATTAT9450.024.4075471
GTGTTAC12750.024.3088381
ACCGTGT9200.024.260488
GCGTAGT3301.900844E-924.0261781
TACCCGT2401.6993708E-623.9996155
GTGTTAG21400.023.9139081
GTATAGG6650.023.8455311
GTCTTAC9800.023.5358491
GTATTAG8700.023.1976911
Produced by FastQC (version 0.11.5)