FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816898
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.037.9836358
GTCTCGC12350.033.8450551
CTGTCGC14000.031.8851959
TCGCTCT13950.030.967244
GTCTTGC25450.030.5823021
GTATAAG8350.027.6183471
CGCTCTG16250.027.4703245
CTCGCTC15950.027.0841983
TCTCGCT15050.026.8112012
GTGTAAC9050.023.8894881
GTCTCAC32850.023.6931381
GTACTAG6150.023.43631
ATACTGA13550.023.3796946
GTATATA13000.022.7287041
GTGTTAG19650.022.3718281
TTGCTCT37950.022.0075474
GTATTAG9000.021.6199871
GGGTTTC49450.020.6945631
GTCTACG2455.360056E-520.5904661
GTCTAGA14050.020.5171891
Produced by FastQC (version 0.11.5)