FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483502
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9450.043.4282958
GTCTCGC9700.042.3158261
TCGCTCT11100.042.1618964
TCTCGCT11500.033.813932
CTGTCGC12700.032.8816839
CTCGCTC12600.031.433443
GTCTTGC21250.027.787831
CGCTCTG12950.026.687095
GTCTCAC25900.023.9110371
GGTATAC4550.023.7399391
CGCTCTA1902.2998119E-422.7366985
GTACCGG1650.002706161721.8215581
GTATAAT5750.021.2902531
GCGCTAG1700.003217882221.1797491
TTGCTCT27700.021.0540184
GTATAAG6900.020.8727971
ATCCGTG2806.47281E-620.5712997
GTGTAGA10650.020.284831
GTCTAGC10300.020.2749841
GGAGTGC50650.020.185465
Produced by FastQC (version 0.11.5)