FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483502
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9900.034.9086388
TCGCTCT10800.031.9995824
GTCTTGC20300.031.5953521
GTCTCGC10100.030.681481
CGCTCTG12650.027.8889665
CTGTCGC13150.026.828559
CTCGCTC12000.025.7996643
TCTCGCT12400.024.4064332
GGCTGGA71100.023.9205761
GGAGTGC53050.023.750875
GTACTAC3102.580964E-823.2470321
GTACGAT1550.001873576723.2470321
GTCTCAC23900.022.9163231
GAGTGCA58100.022.5539236
GTCTAGA10300.022.3893761
TTGCTCT29000.022.0962644
CTGGAGT64000.021.9372143
GTATTAT6150.021.092431
AACCGGT2053.844401E-421.0728996
GTACAGG10750.020.7817671
Produced by FastQC (version 0.11.5)