FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099173
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT25330.12066656726244097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8950.034.5952078
GTCTCGC10250.032.315021
TCGCTCT9600.032.2528234
CTGTCGC11700.028.310179
CTCGCTC11450.028.299423
TCTCGCT11950.027.1153432
GTCTTGC18000.026.8023431
CGCTCTG11450.026.412795
GGAGTGC51450.023.2323535
GTATTAG5000.023.0420171
GTGCTAT7000.022.6305521
GTATTGG6050.022.613551
TACCCGT1600.00226407822.5019665
TTGCTCT25450.022.3516624
GAGTGCA54500.022.3285586
GTCTCAC20400.021.5312961
TCTGTCA26100.021.2432388
TCTCGAT8950.020.9180327
TTACACT6550.020.8873254
GTGTTAC7000.020.5732291
Produced by FastQC (version 0.11.5)