FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099173
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA4200.032.573671
TCTGTCG10000.030.2399228
CCGTCGG1703.1778745E-629.6505139
GTCTTGC19050.029.4823441
ATACCGT1255.3689216E-428.7999256
GTCTCGC10550.027.3004571
TCGCTCT9600.025.4999334
GTCTCAC22900.025.4690021
GTTTAAC6650.024.9039691
GTACGTG2051.3615445E-524.5870571
GTGCGAG3602.0190782E-1024.0016521
CCGTTAT1500.00155566124.0016521
GTCTAGC7700.023.3782311
GTATAGT4353.6379788E-1223.174011
GTGTACA7800.023.0785121
CTGTCGC14800.022.8675279
GTGTTAG11600.022.3463671
GTGTTAC6450.022.3271181
GGCTAGA11300.022.302421
GTGTAAA9400.022.2142941
Produced by FastQC (version 0.11.5)