FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709711
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.040.7588048
GTCTCGC11750.037.993191
CTGTCGC14650.033.4231459
GTCTTGC24500.029.6829621
TCGCTCT12500.029.3789444
GTATTAA8400.026.5726221
GTCTTAC10600.026.4917551
TCTCGCT14150.026.460552
CTCGCTC13500.026.1345043
TTCACCG7700.025.2492855
ACCGTGT8600.024.2815048
GTCTCAC30700.024.1574361
TAATACT7800.024.0024054
TACGCTT1500.001555493324.0024035
GCGTAGA2401.6984031E-624.0010761
GGAGTGC62250.023.9445695
GTATTGG8800.023.7283381
CGCTCTG14150.023.4087075
GAGTGCA69500.023.208086
GTGTTAC10000.023.0410331
Produced by FastQC (version 0.11.5)