FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709711
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12850.037.5429468
GTCTCGC12700.037.4221651
TCGCTCT14350.033.6185954
GTCTTGC26900.032.390811
CTCGCTC15100.030.0425133
CTGTCGC16950.029.739469
GTACTAT5450.029.0679821
TCTCGCT15900.028.9838142
GTACCGT1555.76447E-527.8745771
CGCTCTG16200.027.5570895
GTGTAAC7300.026.6335831
GTGTTAC9500.025.7717551
GTGTTAG16800.025.2889861
ACCGTGT8450.024.7114338
GGATACG2701.7630009E-724.0031071
GATACGC2101.6408341E-524.002222
TTCACCG7950.023.548485
GTATTAT8050.023.2576681
CGACCCG1550.001882993423.2270985
GTATTAC5300.023.0973281
Produced by FastQC (version 0.11.5)