FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423929
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10750.051.57398
CTGTCGC13150.042.1611759
GTCTCGC10750.040.8597761
TCGCTCT12100.036.8938564
TCTCGCT13200.032.7304462
GTCTTGC25400.032.0346031
CTCGCTC15750.029.2599813
CGCTCTG13950.027.35585
TTGCTCT31000.027.1751464
TCGATCT9200.026.609639
GCGCTAG1658.82353E-526.1843591
ATAGTAC4950.026.1843573
GTATTAA6450.025.676911
GTGTTAG17100.024.8445151
GGTATAC4350.024.8299941
CTCGATC9050.024.6638498
GTGTTAA9550.024.1279951
GTGTAAC6950.023.8296491
TCACTCT32450.023.5200864
GTCTCAC30400.023.4496421
Produced by FastQC (version 0.11.5)