FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423929
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.037.3579988
GTCTCGC11500.036.3133161
GTCTTGC22200.033.081331
TCGCTCT12850.030.8167134
GTACTAG5350.029.6076981
CTGTCGC15450.027.9631049
TCTCGCT14150.026.9678422
GTATTCG1358.4192044E-426.6668641
TAATACT6550.026.3813324
GTGTTAC9400.026.0427471
GTATTAA7500.025.9201951
CGCTCTG14300.025.6779825
CTCGCTC14800.025.2969633
GTATAAG6850.025.2264671
GTTTCAC36750.025.0772193
GTACTAT4201.8189894E-1224.000181
GGGTTTC41350.023.680951
TTGCTCT32000.022.2747064
GTACTAC5600.021.8573061
TTCACCG7250.021.847995
Produced by FastQC (version 0.11.5)