FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2545336
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12750.046.3049628
GTCTCGC13000.038.7715071
GTCTTGC24100.037.346591
CTGTCGC17050.036.7418829
TCGCTCT13550.036.6634754
CGCTCTG15450.031.6886885
TCTCGCT15800.030.0759432
CTCGCTC16100.029.0683163
GTATTAG8700.028.9672161
TATACGG1000.00628573628.7994275
ACCGTGT8250.027.9267188
CGTTTCG1358.415837E-426.6687559
TTGCTCT34550.026.0486874
GTCTCAC31650.025.7076681
GTATAAA9650.025.3693641
TACACCG2351.4147627E-624.5101495
CTTGCTC31550.024.4183773
GTTTCAC40650.023.9114323
GATTAGC4650.023.227171
ATAACCG1550.001883789723.2253445
Produced by FastQC (version 0.11.5)