FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341450
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.046.3633278
GTCTCGC11600.039.7312471
TCGCTCT11900.039.3297274
CTGTCGC13950.036.6470229
TCTCGCT13450.034.8017652
GTCTTGC21250.033.5495261
CGCTCTG13150.031.2107095
CTCGCTC13850.030.6769663
GTCTTAC7800.029.5437471
GTATTAG5950.027.8369121
TAACACG4450.027.5070114
TAATACT6600.027.274114
GTATAAT5850.027.0817681
TTGCTCT30350.026.334124
GTGTTAC8900.025.8922711
GTCTCAC28550.025.2234251
GGTATAC4051.8189894E-1224.8933411
GTATTAA6950.024.8677581
GTGTTAG15650.024.3877491
GTATTAC4500.024.0042921
Produced by FastQC (version 0.11.5)