FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378247
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9850.046.7815978
GTCTCGC11750.042.2842861
TCGCTCT13950.037.677314
GTCTTGC22850.035.6090241
CTGTCGC12750.034.4469649
TCTCGCT14700.034.2884982
CTCGCTC15450.031.6918963
GTATTAC5300.029.8892231
CGCTCTG15350.029.5504065
GTGTAAA11950.028.9228531
TAATACT7300.028.6026614
TTGCTCT29700.025.4544754
GGTATAC5450.025.1029591
GTATTGG7250.024.8296071
GTCTTAC10450.024.8058451
TTCACCG6750.024.5332645
GTGTAGT12200.024.198691
GTTTCAC35100.023.5916613
GTCCTAT6250.023.0418721
GTGTTAG16950.022.9399191
Produced by FastQC (version 0.11.5)