FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166018
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9350.046.979078
GTCTCGC8400.040.2944151
CTGTCGC11300.038.234819
TCGCTCT9650.038.056524
TCTCGCT10750.031.4858672
CGCTCTG10850.030.5291185
GTCTTGC19600.030.1289561
CTCGCTC11650.029.6716423
GTCTTAC7550.027.6616021
TACCCGT1607.1552495E-527.0033385
CGTTTCG1500.00155518124.0029669
ATACCCG1851.9179657E-423.3542394
GTACTAT3102.5996997E-823.2308241
GTTCCGA1902.2962218E-422.7417531
GTACAGA10400.022.158631
GTGTAAA9900.021.8228951
GTGCTAT8850.021.157111
GTACTCG2053.838244E-421.0777231
TTGCTCT28100.021.0132734
GTATATA6550.020.8900071
Produced by FastQC (version 0.11.5)