FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2535068
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9800.044.819278
GTCTCGC11900.035.702641
CTGTCGC14250.034.3628659
TCGCTCT13550.031.3526134
GTCTTGC20700.029.917371
CGCTCTG13500.029.3352625
TAGTCCG1000.006283103528.8018935
GTATTAA5700.027.7934931
CTCGCTC14300.027.6941283
GTACTAG4700.027.5784571
TCTCGCT14250.027.2860052
GTATGCG1400.00104020425.7180061
GTATAAG6550.025.28611
GTCTCAC25300.024.4778481
GTACGGA1550.001881984523.2291681
GGAGTGC56000.023.1443775
GTGTTAC7550.022.8907281
GAGTGCA61100.022.8623856
TAGGCCG3503.796231E-922.6300585
TAGGTCG1600.002264491722.501485
Produced by FastQC (version 0.11.5)