FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276962
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10200.033.177268
GCGTTAC1352.2455344E-532.003571
GTCTTGC19900.030.3953531
GTACTAT2853.5106495E-1027.7925761
GTATTAA4200.027.4316331
GTCTCAC26450.026.4074841
GTCTCGC10750.025.4540061
GAGTGCA59350.024.9913256
GGAGTGC56400.024.3835585
TCGCTCT12350.023.9034024
CTGTCGC14300.023.6664589
GGTATAC3352.2864697E-923.644431
GGCTGGA77150.022.4937611
CTGGAGT66750.022.328623
TCTGTCA28150.021.9969718
GTGTAAC5600.021.8595831
GTATAAG5151.8189894E-1220.9732131
TGGAGTG72450.020.9694424
TCACTCT31000.020.9037234
GTATAAT4501.4551915E-1020.802321
Produced by FastQC (version 0.11.5)