FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3472386
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14750.040.5108688
GTCTCGC15650.038.0515561
CTGTCGC18150.033.3187079
TCGCTCT18300.029.1124424
GTCTTGC33000.026.6283531
TCTCGCT19350.026.0590762
GGTATAC8400.025.936711
CGCTCTG20400.025.762295
CTCGCTC19650.025.2946413
GTGTTAA16250.021.8985611
GTCTCAC42250.021.6579191
GTACTAG8900.021.2156451
GTGTTAC15650.020.881951
GTGTTAG25750.020.8702371
GTATTGC11200.020.7493651
GTATTAT13050.020.5903681
GGGTTTC60050.020.5593221
GTGTAGG19100.020.151871
GTGTAAT15600.020.017821
GTTTCAC56550.019.871653
Produced by FastQC (version 0.11.5)