FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493556
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10400.043.6111878
CTGTCGC13200.034.905739
GGCGTAC1251.24890175E-534.8685071
GTCTTGC22150.032.1398931
GTCTCGC11250.031.6399381
TACACCG2105.307138E-727.4259325
TCGCTCT12550.026.9615484
TCTCGCT13200.025.6596222
GTATTAA9000.025.0213811
GTACCGT1450.001278176924.8251976
GTGTTAC10250.024.8048321
GGGTTTC44700.024.051731
GTACTAA4550.023.9481491
GTTTCAC39350.023.8081113
GTATTAG9600.023.4575461
CTCGCTC14250.023.2631933
GTATTGC7200.023.2053151
GGTATAC5950.023.1968351
CGCTCTG14300.023.1586115
GTCTCGA23800.022.38446
Produced by FastQC (version 0.11.5)