FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493556
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11250.041.6625251
TCTGTCG10600.040.7488
TCGCTCT14600.035.5014
CTGTCGC14050.032.2787329
CGCTCTG13900.031.5919175
TCTCGCT14250.031.3626632
GTCTTGC24650.029.7099021
CTCGCTC15800.027.792893
CTTATCG1607.170713E-526.995019
GTGTAAC8350.026.3119431
GTATAGG6750.026.0390781
GTATTAC6300.025.5740971
GTCTTAC10500.025.1091121
GTCTCAC28850.024.8770241
TCCGTAC1550.001885366523.221983
GTTTCAC42850.023.1840463
GTATACG1600.002052144122.885911
GTCTAAG8700.022.7280771
GGGTTTC43350.022.6377811
GGTATAC5550.022.4323141
Produced by FastQC (version 0.11.5)