FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432561
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14400.037.4919478
GTCTCGC15750.036.7505261
TCGCTCT16700.032.3283844
GTCTTGC32850.028.5490461
CTGTCGC18700.028.485869
TCTCGCT19750.027.7422
GTATAAC6750.026.0510041
CTCGCTC19950.025.9793823
CGCTCTG19900.025.6829265
GTATTAG11050.025.1963921
TTCACCG10250.023.1755085
GTACGAC1600.002268348622.4951693
ACCGTGT11500.021.9083378
GTATTGG12750.020.6875631
GCGTAGA3559.094583E-820.6391
GTATAAG9950.020.6182581
GTATTAA10350.020.5293251
GTCTTAC13300.020.3829521
TAGTACT7450.020.2909434
GTACCGA2155.3088745E-420.0887096
Produced by FastQC (version 0.11.5)