FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3423307
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14250.040.416438
CTGTCGC17600.033.9506659
GTATTAC8800.029.7198771
GTCTTGC32750.028.615781
GTCTCGC14700.027.6756531
GTATAAT10850.027.4524611
GGTATAC6500.026.8240911
GTGTAAA20500.024.0980661
TCGCTCT16450.023.1949184
GTGTATA20600.022.9230961
TCTCGCT17700.022.7990362
GTGTAAT12000.022.399981
GTGTTAG26150.022.225191
GTCTTAC13850.022.0306171
GTATAGA9950.021.9040971
GTCCTAC11650.021.8257541
GTACTAG7350.021.7451551
GTGTAGA17400.021.7110731
GTGTAAG17100.021.6671221
TTGCTCT43900.021.3187484
Produced by FastQC (version 0.11.5)