FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3423307
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15850.035.8785178
GTCTCGC15250.032.1565361
GTCTTGC33150.031.573071
CTGTCGC19400.030.4262759
TCGCTCT17600.030.2664894
GGTATAC7550.030.0523931
GTGTTAC15600.028.6199951
CTCGCTC18450.027.7016093
GTACTAC6850.025.6439531
ACACCGT5650.025.4814766
TCTCGCT19100.024.9046462
GTCTTAC12650.024.8795361
CGCTCTG19250.024.680635
TACACCG2651.4766738E-724.4477925
CGTTTCG1500.00155848523.9947059
GTATTAG12250.023.8994671
TTACACT17650.022.8394854
GTACCGG2903.0028423E-722.7147961
GTATAGA10700.021.8892331
TTGCTCT47050.021.8785954
Produced by FastQC (version 0.11.5)