FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470683
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG1001.4517104E-435.9927529
GTCTCGC13900.032.680691
TCTGTCG14150.030.5238888
CTGTCGC17450.025.5765139
GTACGGA2401.4597426E-624.4226671
GTTCTAC9700.024.1708891
CGGTACT1500.00155831423.9951694
GTATTAC8000.023.8121031
TCGCTCT15900.023.542434
TCTCGCT17350.022.0208992
GTATTAG10750.021.8100091
GTGTAAC9500.021.594781
CTCGCTC16500.021.3772073
CCGTTAG1750.0034436720.9337161
GTCTTGC28800.020.6066271
GTTAGCG2104.4866174E-420.5963692
GTATTAA8900.020.5808981
GTATAAC6100.020.4189511
GGAGTGC66650.019.9809745
GTACGAA2256.401768E-419.5381361
Produced by FastQC (version 0.11.5)