FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387938
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14700.036.241178
CTGTCGC17700.032.5390249
GTCTCGC18150.032.0214921
TCGCTCT18450.031.217224
CTCGCTC19700.028.8927273
TCTCGCT19600.028.3053632
CGCTCTG19500.027.6898655
GTCTTGC33900.027.6451031
GTGTAGA16500.024.6565511
GTACCGG2852.728375E-722.9417151
GTCTCAC42200.022.5521051
GTCTTAC13600.022.4356481
GTATAAG9300.021.8727471
GCGTTAG2353.654298E-521.6400571
GTATAAT9700.020.9707781
GGTATAC6450.020.2740751
GTGTTAG24750.020.2535951
GTGTAAC11850.020.23131
CTATCCG1800.004487157319.9985314
TTGCTCT46950.019.9346394
Produced by FastQC (version 0.11.5)