FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820560
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11200.045.6336638
CTGTCGC14650.037.3440179
GTCTTGC26800.036.0810931
GTCTCGC13850.033.850941
GTGTTAC11150.030.8790231
TCGCTCT13650.030.5867044
TCTCGCT16600.026.4891322
CGCTCTG16450.025.3804555
GTGTAGC14250.024.1614841
CTCGCTC17600.024.1310733
GTATTAG9550.023.7792911
GGTATAC4403.6379788E-1223.3085841
TTGCTCT38000.023.3001564
TCTTGCT40400.023.1957242
TTCACCG7450.023.1895475
CTTGCTC36200.023.0667693
GTTTCAC42450.023.0620843
GTACAGA14950.022.540171
GGGTTTC46850.022.5161171
GTACTAA6250.022.269691
Produced by FastQC (version 0.11.5)