FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856301
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10550.042.303828
GTCTCGC11700.038.812961
GTCTTGC26950.033.9720841
TCGCTCT13300.033.0155534
TCTCGCT13650.031.6799032
CTGTCGC14700.031.3397439
TACACCG2202.4301698E-829.44835
CTCGCTC15350.028.1373543
GCGTAAT1409.4208634E-426.1585061
GTGTTAG20250.025.6805481
GTACGGA2009.851894E-625.6353341
CGCTCTG16100.025.0381685
TTGCTCT37700.024.6313784
GTATTAG8150.024.2648221
GGAGTGC69050.023.3520325
GGGTTTC46550.023.286971
GAGTGCA74850.022.6004146
GTGTAAC9200.022.2915971
GTACTAC5300.022.1113411
TCTTGCT40850.022.0537872
Produced by FastQC (version 0.11.5)