FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955886
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11850.042.5271848
GTCTCGC13450.038.338081
TCGCTCT14100.036.7633444
CTGTCGC14750.035.6301579
TCTCGCT16450.032.4136242
CTCGCTC15600.030.9460073
CGCTCTG16750.029.2272785
GTCTTGC28700.028.0889431
GTCCTAC9650.027.8463761
TACACTG17300.026.2173445
GTGTTAC11400.025.4829351
GTATAAA12700.025.161891
GTCTCAC34000.024.9920121
GTTTCAC43850.024.3190443
GTGTTAG22100.023.6610771
TCCTACG3352.2700988E-923.6597232
GTGTAAG15450.023.5036771
TAGTACT6850.023.1224444
GGGTTTC52250.022.795641
GTACAGA17850.022.3778721
Produced by FastQC (version 0.11.5)