FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429983
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11300.043.322588
GTCTCGC10300.037.3750841
CTGTCGC13800.037.039339
GTATTAT8750.029.0538391
GTATTAG8500.028.1993141
TCGCTCT13050.027.5836834
GTCTTGC24200.027.0128691
TCTCGCT13350.026.984372
GTGTAAC9050.025.6829531
CGCTCTG13100.024.7300555
CTCGCTC14350.024.6018493
TAATACT8650.024.136524
GTGTAAA15050.023.1658521
GTACGGT1902.1670255E-422.9372431
GTATAAC5150.022.566091
GGGTTTC43000.022.2971321
CTCGATC8800.022.0884348
TTCACCG8150.022.0834165
GTTTCAC38000.021.9936833
GTCTCAC30650.021.5652471
Produced by FastQC (version 0.11.5)