FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429983
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9950.044.8563738
GTCTCGC10350.038.2343751
TCGCTCT12850.033.612724
CTGTCGC13800.033.3853879
GTGTTAC12200.033.037221
GTCTTGC25600.031.7748581
CGCTCTG14000.028.7948975
ATACCGC1255.3746556E-428.7948976
TCTCGCT13200.027.8579652
CTCGCTC15400.027.1120783
GTATTGC6650.024.2438531
GTACTAC3952.0008883E-1124.118311
GTACGGT1851.7031319E-423.7673171
GGGTTTC43150.023.4368321
TTGCTCT34750.023.40884
GTAGTAC4353.6379788E-1223.1683083
GTGTAAC7450.022.6241461
GTTTCAC39550.022.2059253
AATGGCG13300.022.1915575
GTATAGC5350.021.9162791
Produced by FastQC (version 0.11.5)