FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407159
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7700.044.878788
GTCTCGC10200.038.3353351
TCGCTCT11350.034.3049244
TCTCGCT11800.032.3937262
GTCTTGC18450.030.612861
CTGTCGC11250.030.0770939
CTCGCTC12600.029.1849023
CGCTCTG10600.027.8463675
GTACTAA4051.8189894E-1225.0310271
GTCTACG1801.53385E-424.1370641
GTTCTAT5700.024.1370621
GTTTCGC4550.023.7705083
GTATTAC4650.023.3584481
GTGTTAG13600.022.362281
TTCCGAA1952.7104287E-422.1913492
GTGTAAA11200.021.9819681
GTACTAT4007.021299E-1021.7233561
GTACTCG2353.7472208E-521.569291
TCTGTCA24400.021.5389048
GAGTGCA52900.021.3665546
Produced by FastQC (version 0.11.5)