FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240180
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9150.040.914978
GTCTTGC21650.036.427211
TCGCTCT11300.034.4483344
GTCTCGC10750.033.6526221
CTGTCGC12950.033.3565759
CTCGCTC12400.031.3924333
CGCTCTG12700.029.4781065
TCTCGCT13350.027.542292
TTGCTCT31350.025.7533264
GTCTCAC25150.024.7410341
GTATAAT5950.024.3203811
GTTTCAC34100.024.3106373
GTGTAAC6850.024.2937551
CTGTTGC25400.024.0926849
CGTTTCG1500.001556995623.9982039
GGGTTTC37400.023.988741
TCTGTTG25100.023.5201538
GTGTTAG17050.022.9153611
GTGTATA12000.022.9118251
TTATACT6650.022.7641034
Produced by FastQC (version 0.11.5)