FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240180
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10200.044.4759038
GTCTCGC8950.042.155561
TCGCTCT11200.036.643154
GTCTTGC20550.032.835651
GTATTAG6750.030.0973871
CTGTCGC16300.030.0404019
GTATTCG1453.4738732E-530.0232561
TCTCGCT12300.029.8645612
CGCTCTG13800.028.1741375
GCGTTAG1907.1176128E-626.7312361
TTGCTCT31350.026.4116924
CTCGCTC13100.026.3771783
GTGTTAT8000.025.394671
GTCTCAC25250.025.2869131
TTATACT6450.024.5583344
GTATAGG6000.024.18541
CTTGCTC28050.023.8675823
GTACTAG4700.023.1562351
GGGTTTC37850.023.0032861
CTGTTGC25650.022.739569
Produced by FastQC (version 0.11.5)