FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252528
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9000.042.3949668
GTCTTGC22000.041.117811
CTGTCGC12250.036.436499
GTCTCGC10500.033.7714271
GTATAAC3450.029.3664591
GTCTTAC7650.029.3253291
TCGCTCT11350.029.2134694
TCTCGCT12900.027.947712
GGTATAC4550.027.038351
TTGCTCT29450.026.6785584
GTCTCAC26700.026.5617981
GTATTAC5450.026.5568241
GTACGAC1100.00995124126.2112923
CGCTCTG13000.026.027685
GTATTAA5850.025.9780221
GGGTTTC38600.025.3098221
TAGTACT4600.025.071674
GTATTAT6950.024.9901621
TCTTGCT32650.024.9552082
GTTTCAC36000.024.2272433
Produced by FastQC (version 0.11.5)