FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252528
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9700.035.149761
TCTGTCG10300.032.8588688
GTCTTGC21600.030.2263031
TCGATCT9550.027.1450799
TCGCTCT11000.026.8388464
GTATTAA6050.026.3793181
GTCTTAC9100.026.3068491
TCTCGCT11950.025.9155272
CTCGATC9750.025.8496938
CTAATAC5800.024.8260253
TAATACT6100.024.7891794
GTACTAT4750.024.4355771
GTCTCAC27800.023.4851841
GGGTTTC37400.023.4698351
GTTTCAC33750.023.4651893
CTCGCTC13500.023.4651893
TCACTCT30000.022.0820434
CGCTCTG14100.021.9594785
GTATTGC5950.021.9458181
GTATTAC4307.094059E-1121.9316431
Produced by FastQC (version 0.11.5)