FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240000
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8850.043.115418
TCGCTCT9350.038.5471084
GTCTCGC9250.037.5555231
GTCTTGC19800.036.1863141
CTGTCGC12000.032.397579
CGCTCTG10300.030.7542885
GTGTAAC6600.030.7035391
CTCGCTC10800.029.367193
TCTCGCT11650.029.0872332
GTCTTAC8450.028.2638651
GTGTTAC9550.025.766171
GTGTTAG16250.025.386091
GTTTCAC32600.024.543633
TTCACCG6200.024.3847225
ACCGTGT7300.023.6694568
GGGTTTC39350.023.1739541
TTACTCT11000.022.935534
GTATTAT6650.022.8545131
CTTGCTC27800.022.817673
GAGTGCA58350.022.3320166
Produced by FastQC (version 0.11.5)