FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054037
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8900.053.3874178
CTGTCGC11150.042.614179
GTCTCGC9250.041.4817921
GTCTTGC19950.041.0070881
TCGCTCT9150.038.607674
TCTCGCT11100.034.4290962
GTACTAT4450.034.1650961
CGCTCTG11250.030.7166425
TTGCTCT28850.029.987074
CTCGCTC11900.029.6857283
ACTGTCG2105.3069925E-727.4255718
CTTGCTC28750.026.8314743
GGTATAC3900.025.9888341
GTCTAAC5150.025.3039681
GGGTAAG10100.025.088231
GTATAAA8200.024.7210871
GTATTAT5900.024.5415131
TCTTGCT32400.024.0354042
GTCTCAC23300.023.9253431
GTGTAGA11350.023.6009371
Produced by FastQC (version 0.11.5)