FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377856
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11600.039.408261
TCGCTCT12700.034.014884
TAGTCGA850.00283481933.8886152
TCTGTCG11450.031.4415688
CTCGCTC14250.031.322883
GTCTTGC22300.030.2609671
TCTCGCT14350.029.6082572
GGTATAC4500.027.4120251
CGCTCTG15200.026.5256395
GTATACG1701.02597114E-425.609851
CTGTCGC15450.025.1654629
TAATACT6900.025.0428374
GTCTCAC29950.024.4697341
GCGTTAC1500.001487542124.187081
GGGTTTC39250.024.0330241
GTAATAC5450.023.7772083
GTATTAA6850.023.3043421
GTACTAT4053.092282E-1123.2912621
GTTTCAC35600.023.2558773
GTGTTAG15800.022.962421
Produced by FastQC (version 0.11.5)