FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206698
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9450.044.9481058
GTCTCGC9050.041.60191
CTGTCGC12500.038.012399
GTCTTGC21900.037.689391
GGTATAC4200.032.7538721
TCTCGCT10200.031.106622
GTATTAA5800.028.7116531
TCGCTCT10050.028.6956084
GTTTCAC32850.025.6786383
TTGCTCT27050.025.5873584
CTCGCTC11350.025.4088843
GTGTATA12300.025.3117221
TAGCGGT2001.1132433E-525.2341984
GGGTTTC36600.024.7279031
GTATTAT6150.024.7230781
GTGTAAA11500.024.5541631
CTTGCTC27600.024.032573
TAATACT4850.023.7848114
GTGTTAG14450.023.5498621
CGCTCTG13150.023.5414895
Produced by FastQC (version 0.11.5)