FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206698
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10700.048.8072171
TCGCTCT10100.047.764734
TCTGTCG11100.045.4117628
CGCTCTG12950.038.3648265
GTCTTGC19100.036.4563521
CTCGCTC13300.035.7254143
TCTCGCT14150.035.620132
GTGTAAC5750.031.5360661
GTGTTAG15950.028.6493781
GTCTTAC6350.028.5562781
CTGTCGC17250.028.3865819
TAATACT6100.027.148884
GTCTCAC24500.026.052651
TTACACT9200.025.8273584
GTATAGG4550.025.5060921
GTATTGC4850.025.4239221
TTGCTCT24300.024.8901164
GTATTAG6750.024.7149311
GGGTTTC35450.024.5527611
GAGTGCA57000.024.0017246
Produced by FastQC (version 0.11.5)