FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959127
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11850.041.919588
CTGTCGC15450.034.480599
GTCTCGC13700.032.407641
TCGCTCT14550.030.6901044
TCTCGCT15350.026.2811742
CGCTCTG14550.026.2240055
CTCGCTC15700.026.151043
GTCTTGC24000.025.7777941
GTCTCAC29350.025.0467471
GTGTAAC7350.024.7565861
GTCTTAC9150.024.6591841
GTGTTAG16900.024.5485731
GTATAAC4103.6379788E-1123.0779691
GTCTAAG9350.022.574831
GTATTAT6650.021.8900341
GTATTAA7750.021.600521
TTGCTCT33550.020.8232674
GTCGTAA1750.00357829720.7955341
GGCTACG2856.869117E-620.4306981
GTATAGT6450.020.3119161
Produced by FastQC (version 0.11.5)