FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959127
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12100.031.1689551
TCTGTCG11250.030.713338
TCGCTCT14350.028.5935754
GTCTTGC22050.026.1591421
TACACCG1701.08311986E-425.4066755
CTCGCTC15250.023.6026253
CTGTCGC17150.022.2458979
GTCTAAC5051.8189894E-1221.965311
TCTCGCT16450.021.9008392
GGAGTGC64200.021.8647795
GTGTAAA16400.021.6438661
GAGTGCA68050.021.2624576
GTCTACG2103.7767604E-421.1285381
GTACTAT5001.8189894E-1120.7059651
GTACAGA11500.020.5773581
CGCTCTG15050.020.5673085
TTGCTCT34100.020.4768354
TCTGTCA33500.019.9837198
GGGTAAC8650.019.6629751
GTATTAA7150.019.6510161
Produced by FastQC (version 0.11.5)