FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357940
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.044.9493338
CTGTCGC12300.039.2136089
GTCTCGC11650.036.23661
TCTCGCT12950.031.1429962
GTCTTGC23150.031.1264481
TCGCTCT12000.030.0037544
GTACTAT6450.028.2114661
TACGACT1358.4133475E-426.6700064
GTATTAG8000.026.3847711
GTGTTAG16050.026.3025761
CTCGCTC13800.025.568963
GTATTAC5750.025.3167231
GTATAGA8050.025.3167211
GTACTAG4650.025.0444981
GTGTAAT9400.024.0037561
CGCTCTG13450.023.550365
GTATTGA7750.023.4792211
GTGTAAG12400.023.4792211
GTAATAC6150.023.4180623
GTTTCAC37000.022.9655253
Produced by FastQC (version 0.11.5)