FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1949964
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8950.037.0013548
CTGTCGC11150.034.865979
GTACGGT1102.4000501E-433.040391
GTCTCGC9350.032.651681
TCGCTCT9400.031.4151674
GTCTTGC19850.029.2952561
GTATTAT5900.025.8723051
GTATTAG6600.025.3309631
GTGTAGA9550.024.3564761
TTGCTCT27600.024.0083374
CTCGCTC10850.023.8983143
TCTCGCT11900.023.6090742
GTCGTAT1550.001781922123.4480171
GTACTAG3752.9831426E-1023.2604331
GTATAAT5950.023.2115651
GTCTCAC24200.023.1282711
GTGTAGG13050.022.8371121
TAATACT6100.022.4340214
CGCTCTG12550.022.3719445
GTATAGG4306.91216E-1121.97571
Produced by FastQC (version 0.11.5)