FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1949964
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9350.034.6456458
GTCTCGC8800.033.5449141
TCGCTCT10200.033.1700024
GTCTTGC18450.032.399481
CGCTCTG11600.029.1667275
CTGTCGC11400.029.0469679
GTGTAGG11100.026.5941641
GTGTTAG11800.025.0165441
TCTCGCT11900.024.8216152
CTCGCTC11950.024.6981123
GTGTAAC5400.024.5996021
GTCTCAC24550.023.1466731
GTGTTAC8350.022.9792691
GTATAGT5700.022.010171
GTACGGC1650.002711454221.8139293
GTTACAC6650.021.6499143
TCTGTTG21400.021.5285118
TTGCTCT26300.021.349454
GTCTTAC8300.021.3394151
GTGTAGT10850.021.0853751
Produced by FastQC (version 0.11.5)