FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447258
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11100.042.1557778
TCTATCG1901.4006218E-1037.8890048
CTGTCGC13650.035.86279
GTCTCGC11100.030.1480751
GTCTTGC25150.027.1903361
GTATAAT6450.027.069251
GTATTAC7050.026.8292751
TCGCTCT12550.026.3950734
TTACACT13250.025.5441064
GTATTAT7450.025.3887791
GTGTTAC10750.025.0390591
GTGTTAG17450.025.013851
GTATTGC7600.023.9304681
GTCTCAC33400.023.7413141
CTCGCTC13650.023.7409173
GTACTAG3702.4920155E-1023.5941471
CTATCGC2151.9721681E-523.4383139
TAATACT7100.023.328044
GTATAGG6950.023.0283371
TCTCGCT13800.022.4433082
Produced by FastQC (version 0.11.5)