FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848983
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11750.045.3364458
CTGTCGC16700.033.6226359
GTCTCGC13050.033.3896831
GTCTTGC28450.032.7083781
GTACGGA2059.5205905E-932.4234661
TCGCTCT13100.032.4215934
CTCGCTC15950.026.1770633
GTGTTAG19850.026.0440371
TCTCGCT16600.026.0339932
GTATTCG2309.3984636E-725.6881561
GTACTAT5500.025.513011
GTATTAG8800.025.1773131
GTGTTAC11200.025.057421
TATACTG8100.024.8843695
GTTTCAC42300.024.8465523
GGGTTTC47750.024.7467061
GTATTAC6600.024.6178151
CGCTCTG17000.024.5602465
GGTATAC5450.024.3919641
TAATACT7800.023.9956424
Produced by FastQC (version 0.11.5)