FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2264769
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC18600.034.1530881
TCTGTCG10100.033.4982728
GTATTAG3900.032.1979871
TCGACTA900.003757944831.9936245
GTATAAG6150.031.2278921
GTATTAA4500.031.1878551
GTCTCGC10200.029.6912251
GTCCGTA1000.005546351429.546391
GTCCTAC6250.029.5463891
TCGCTCT10800.026.661354
CTGTCGC13600.026.4653139
GTCCTAA5500.025.5173361
GCGTTAC1450.001100390525.4710251
GGTATAC3201.0004442E-925.3914281
GTGTTAT4800.024.6219921
GTCTCAC23800.024.2081761
GTATAGT4301.8189894E-1224.0493871
GTCTAGC6650.023.3260971
GTGTTAG12450.023.1387391
TTGCTCT25400.022.9560534
Produced by FastQC (version 0.11.5)