FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281717
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11550.039.6995161
TCTGTCG10250.038.625588
TCGCTCT13500.033.593294
GTCTTGC21850.033.1703571
CTGTCGC13950.030.4448629
TCTCGCT14600.029.6103842
GTATCGC1000.00551330929.5825421
CGCTCTG14150.029.506475
GTCTTAC9650.028.3563231
CTCGCTC15150.027.0836983
TCTGTCA28750.025.2883058
GTTTCAC37050.025.2581083
GTACTAA5050.024.8961981
GTATAGT4850.024.3979741
GTGTAAT8800.024.371981
GTATTAG8300.024.058091
GTGTTAC9850.024.026431
GGGTTTC39750.024.0009331
GTACTAT4700.023.6030941
GTGTAAA13700.023.2125781
Produced by FastQC (version 0.11.5)