FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2503153
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGA400.00596097554.5835531
GTCTCGC10950.038.5491141
TCTGTCG8400.035.9950758
TCGCTCT10950.032.2257164
GTCTTGC23200.030.1150671
CTGTCGC11300.028.0315659
TCTCGCT13900.027.4648442
CGTTCCG1907.5645385E-626.5226885
GTGTTAG16250.025.9761751
GTATTAC6350.025.214451
GGTATAC4950.024.994491
GTATAAC5400.024.259361
CTCGCTC14450.023.9227623
GTATTAT7050.023.7432021
CGCTCTG13100.023.6303565
GTTTCAC38800.023.3871213
TATACTG8650.023.3031715
GTGTAGA11100.022.9480441
TAGTCCG1902.301852E-422.7337325
GTGTAAT10600.022.6573241
Produced by FastQC (version 0.11.5)